EGFR and neoplasm: To demonstrate that the tumor gene reprogramming promoted by PGE2 was mediated by nuclear EGFR, the expression of EGFR was genetically ablated by CRISPR/Cas9 in A549 (Figure 3A) and GLC82 cells (Supplementary Figure 3A), and then two clones, knockout for EGFR (EGFR −/− #1, #2), were transfected with EGFR plasmids bearing a wild type (WT) or a mutated nuclear localization sequence, NLSm12 and dNLS, respectively [32].