GLYAT and Familial prostate cancer: Here, recombinanat lentiviruses stably transduced castration-resistant prostate cancer cell lines (i.e., PC3, DU145, 22RV1) and an nontransformed prostate epithelial cell line (RWPE1) with tetracycline-inducible expression vectors encoding GFP (control) or Gαt (Figure 1A).