Similar results were observed with a selective inhibitor of Gβγ, gallein, in RWPE1 (not shown) and PC3 cells (Figure 1C), suggesting that GPCR stimulated AKT and ERK phosphorylation through a Gβγ-dependent pathway in prostate cancer cell lines, and both Gβγ-independent and dependent pathways in RWPE1 cells. This evidence concerns the gene AKT1 and prostate cancer.