Moreover, we also designed another siRNA to confirm the effect of down-expression of B7-H6 to the cellular function of glioma cell lines, and the B7-H6 mRNA expression level was also significantly decreased in the siRNA transient-transfection cells in contrast to the si-NC transient-transfection cells (Supplementary Figure 1). This evidence concerns the gene NCR3LG1 and glioma.