To investigate, if B7-H6 has any specific role in the biological regulation of glioma cells, we first knocked down its expression using recombinant lentiviral GFP vector containing, shRNA targeting B7-H6 gene (LV-B7-H6-shRNA virus) and the non-targeted shRNA sequence as control (LV-NC virus). Here, NCR3LG1 is linked to central nervous system cancer.