Next to identify the specific role of B7-H6 in the growth of human glioma cells, we compared the cell proliferation rate of U87 and U251 cell lines infected with either LV-B7-H6-shRNA or LV-NC, using CCK-8 assay As shown in Figure 3A, we observed that after 24, 48 and 72 hrs, the proliferation rate of U87-LV-B7-H6-shRNA cells was significantly lower than that of U87-LV-NC (P<0.05), while U251-LV-B7-H6-shRNA cells showed significantly lower proliferation rate only after 48 and 72 hrs than U251-LV-NC (P<0.05). The gene discussed is NCR3LG1; the disease is central nervous system cancer.