Both the induction and the blockade of the autophagic flux can lead to a decrease in the free GFP signal in western blot (S5 and S6C and S6D Figs) [30], but because the ratio in GFP-Atg8 puncta between cells treated with PI and not treated was lower for an infection with M. marinum wt (Fig 6C), we conclude that only M. marinum wt blocks the autophagic flux. This evidence concerns the gene GABARAPL1 and infection.