In D. discoideum, we detected not only an increase in the number of Atg8+ and Atg18+ structures (Figs 1A and 1B and S1D–S1E), but the recruitment of those structures and other autophagy markers like Ub and p62 to the MCV early after infection (Fig 1C, 1D and 1F). The gene discussed is WIPI1; the disease is infection.