GABARAPL1 and infection: With time during infection (5–7 hpi), the autophagy induction driven by M. marinum diminished, as confirmed by the decrease in (1) the transcription of autophagy genes (Fig 4E), (2) the total number of GFP-Atg8 structures per cell (Mm wt mock in Figs 6B and S7D), and (3) the recruitment of those GFP-Atg8+ and GFP-Ub+ structures to the MCV (Fig 4A and 4B).