Fig 4A depicts the percentage of IL-17 and IFNγ producing T cells in the iv.CD45-ve CD4 populations. The data showed that only i.n. vaccinated mice experienced an increase in the percentage of Th17 T cells early after infection. In contrast, s.c. vaccinated mice showed a small increase in IFNγ producing T cells (Fig 4B). This was a transient increase since it was not observed at day 7 post infection (Fig 3). IgA was also increased, but only in i.n. vaccinated mice (Fig 4C). This evidence concerns the gene CD4 and infection.