In this paper we report a complementary in vivo approach to investigate LAP interactions in the rodent malaria parasite P. berghei, using a series of existing and newly generated genetically modified parasite lines stably expressing LAPs fused to green fluorescent protein (GFP), combined with GFP affinity purification and label-free quantitative mass spectrometry (LFQ MS). This evidence concerns the gene LAP3 and malaria.