In line with this study, we observed that glucose-restricted OVCAR3 Gluc-2 and Gluc-3 sublines had increased expression of ovarian cancer stem cell markers, such as CD44 and CD117 [35], as well as decreased expression of CA125 (MUC16) [38], an ovarian cancer marker negatively correlated with stemness (Supplementary Table 1 and Supplementary Figure 1B). Here, MUC16 is linked to ovarian cancer.