To validate these data and analyze the relationship between ZNF521 expression and distinct MLL-fusion genes, we performed quantitative real-time PCR (qRT-PCR) in an independent cohort of 50 pediatric AML patients (16 MLL-rearranged and 34 non-MLL-rearranged; Supplementary Table 1) and 7 normal bone marrow (BM) controls. The gene discussed is ZNF521; the disease is acute myeloid leukemia.