Upon neutralization of IL-10 in DC cultures stimulated with R848 in the absence of ascites (R848 +αIL-10) the expression of the monitored DC activation markers (CD86, IL-6, IL-12p40 and TNFα) was consistently higher than observed for DC stimulated with R848 in the presence of ascites and IL-10 neutralizing antibody (R848 +AF +αIL-10) even though some of these results were not statistically significant (Fig 6A). This evidence concerns the gene TNF and Ascites.