Therefore, to study the impact of CysLT1R in colitis-associated colorectal cancer, we employed the azoxymethane/dextran sodium sulfate (AOM/DSS) model in mice with disrupted expression of CysLT1R. This model is a two-step model where the tumor development in the colon is initiated with the carcinogen azoxymethane and then promoted by the administration of the irritant dextran sodium sulfate [26]. Here, CYSLTR1 is linked to colitis.