CASP3 and glioblastoma: To summarize, despite caspase 3/7 activation, Vac-inducible cell death at 7 μM did not lead to an apoptotic phenotype, which includes nuclear fragmentation; instead, glioblastoma cells died by vacuolization and eventual rupture of the plasma membrane (Figures 3C, 3D; Supplementary Figure 2), facilitating rapid PI uptake (Figures 1A, Supplementary Figure 2).