Next, to examine whether nuclear accumulation of IGFBP2 is present in mTORC1-active cells, we performed double immunofluorescent staining of LAM specimens from two LAM subjects (LAM-1 and LAM-2) with IGFBP2 and phospho-S6, an indicator of mTORC1 hyperactivation. This evidence concerns the gene IGFBP2 and lymphangioleiomyomatosis.