Although both SW480 and SW620 cells are separated from primary and lymph node metastasis foci of the same CRC patients, the inconsistent expression of Cyclin B1, AIF, PI3K, Beclin 1, LC-3B, and β-catenin in BTG3 might underlie the molecular mechanisms of the differences in the biological behaviors of SW480 and SW620, even their BTG3 transfectants. The gene discussed is AIFM1; the disease is colorectal carcinoma.