Treatment with the MLCK inhibitor ML-7 and the Ca2+ inhibitor 2APB, but not the ROCK inhibitor Y27632, decreased the punctate staining of E-cadherin in the cytoplasm and the cytoplasm: junction FIR of E-cadherin to or below the control level in epithelial cells without GC inoculation (Fig 5A and 5B). Here, CDH1 is linked to gonorrhea.