APOE and atherosclerosis: To test a direct participation of TLR7 in atherosclerosis, we crossbred TLR7-deficient (Tlr7−/−) mice with apolipoprotein E-deficient (Apoe−/−) mice and produced Apoe−/−Tlr7−/− and Apoe−/−Tlr7+/+ littermates, followed by feeding them an atherogenic diet to produce atherosclerosis.