In order to investigate the significance of Pin1 in sorafenib-induced cell death, we stably knocked down Pin1 expression in Huh7, HepG2 and SK-Hep-1 HCC cells using validated Pin1 shRNA lentiviruses, which led to effective Pin1 knockdown, as compared with scrambled shRNA control cells (Figure 2A, 2D and 2G), as described previously [34]. The gene discussed is PIN1; the disease is hepatocellular carcinoma.