Based on the subgroup analysis by methods, significant association between APC methylation status and NSCLC was found in the MSP and PCR subgroups (OR = 10.32, P < 0.001; OR = 99.10, P = 0.001; respectively), but not in the qMSP subgroup (P = 0.086). This evidence concerns the gene APC and non-small cell lung carcinoma.