To investigate whether there was an inverse correlation between PVT1 and miR-203 in ESCC cancer tissues, qPCR in 104 ESCC cancer tissues indicated a significant inverse correlation between PVT1 and miR-203 (Figure 4E, r = −0.657, P < 0.001). This evidence concerns the gene PVT1 and esophageal squamous cell carcinoma.