Our study indicated that knocking down PTBP1 or suppressing cortactin could both decrease cell migration and invasion in colon cancer cells (Figure 2 and Supplementary Figure 4) and PTPB1 mediates inclusion of the alternative exon 11 in cortactin pre-RNA changing the F-actin binding domain involved in cell motility (Figure 5 and Figure 7D). Here, PTBP1 is linked to colonic neoplasm.