To investigate whether uPA promotes ESCC progression via PI3K/AKT or ERK signaling pathways, we treated EC109 and KYSE30 cells for 30 min with 10 μM LY294002, PI3K inhibitor, or U0126, MEK inhibitor, before uPA or CAF CM treatment. This evidence concerns the gene PLAU and esophageal squamous cell carcinoma.