We further investigated the effects of VEDT on self-renewal (spheroid formation) of pancreatic CSCs L3.6pl CD24+CD44+CD133+ isolated from the pancreatic cancer cell line L3.6pl using flow cytometry and the commercially available patient-derived human (parental) pancreatic CSCs CD24+CD44+CD133+ ESA+ grown in three-dimensional ultra-low non-adherent culture plates containing stem cell medium (Figure 1D and 1E). Here, CD24 is linked to pancreatic neoplasm.