High expression of CtBP2 in GC was significantly associated with differentiation (p = 0.035), TNM stage (p = 0.004), T classification (p < 0.001), lymph node metastasis (p = 0.002), distant metastasis (p = 0.002), vascular invasion (p = 0.004), and lymphatic invasion (p = 0.004). Here, CTBP2 is linked to gastric cancer.