Three major patterns of PD-L1 expression were found in ESCC specimens: (i) a diffuse PD-L1 expression in the presence of TILs; (ii) regional expression of PD-L1 colocalized with TILs; and (iii) PD-L1 expression at the invasive front (Figure 3). Here, CD274 is linked to esophageal squamous cell carcinoma.