To investigate whether the antitumor effect is correlated with the immune responses induced by HPV DC-based vaccine, spleens were collected from the above tumor mice on day 30 to detect the frequencies of CD4+ and CD8+ T cells and their activation status, the frequencies of regulatory T cells (Tregs), macrophage and myeloid-derived suppressor cells (MDSCs), antigen-specific proliferation of splenocytes and CD8+ T cell responses. Here, CD4 is linked to neoplasm.