We find that NUSAP1 has limited effects on proliferation, but rather is associated with development of metastatic disease, possibly through modulation of expression of FAM101B. FAM101B is involved in cell shape remodeling during the epithelial to mesenchymal transition and is a signaling effector of TGFβ1 [33, 34], which promotes invasion and metastatic spread during prostate tumor progression [35]. This evidence concerns the gene RFLNB and metastatic neoplasm.