NUSAP1 appears to drive prostate cancer progression by promoting invasion, migration, and metastasis of prostate cancer by modulating gene expression changes, including modulation of FAM101B. Taken together, our work provides a better understanding of the function of NUSAP1 in aggressive prostate cancers, provides rationale for using NUSAP1 as a prognostic biomarker, and sets the stage for developing improved therapeutic strategies for prostate and other cancers. Here, NUSAP1 is linked to prostate cancer.