NUSAP1 and infection: To gain insights into gene expression alterations induced when NUSAP1 is modulated, we used lentiviral infections to overexpress or knockdown NUSAP1 in DU145 or PC-3 cells for 72 or 96 hours, verified overexpression or knockdown by RT-qPCR and western blot (Supplementary Figures 1A and 1B), and performed RNA sequencing (RNA-Seq).