NUSAP1 and infection: To further determine if NUSAP1 expression would affect proliferation in vivo, we used lentiviral infections to overexpress or knockdown NUSAP1 or controls in PC-3-luc2 cells (PC-3 cells that stably express the firefly luciferase gene), verified overexpression or knockdown of NUSAP1 by RT-qPCR and western blot (Supplementary Figure 1E), and tested whether NUSAP1 overexpression or knockdown affected growth of PC-3-luc2 cells in mouse xenografts.