NUSAP1 and Familial prostate cancer: NUSAP1 binds DNA to the mitotic spindle and we have shown that knockdown of NUSAP1 results in reduced proliferation of DU145, LNCaP, PC-3, and PC-3-RB1 low prostate cancer cells grown in culture [11, 31], yet it was unknown whether NUSAP1 overexpression would enhance proliferation.