Overexpression of NUSAP1 or EGFP control in DU145, LNCaP, PC-3, or 22Rv1 prostate cancer cell lines using a lentiviral vector (Supplementary Figures 1A-1D) did not significantly affect proliferation rates over a period of 5 days (Supplementary Figures 2A-2D). Here, NUSAP1 is linked to prostate cancer.