Similar to the reduction in M1 and M2 macrophages, CRCs with VEGFA amplification or Chr6 polysomy were preferentially associated with the absence of or the reduction in PD-1-positive tumor infiltrating lymphocytes than in VEGFA copy number neutral CRCs (p = 0.0188, Chi-square test; Fig 4A), but were not different from VEGFA copy number neutral CRCs in terms of the number of PD-1-positive stromal lymphocytes (p = 0.3868, Chi-square test; Fig 4B). This evidence concerns the gene PDCD1 and neoplasm.