To understand the immune microenvironment of CRCs with VEGFA gene copy number amplification or Chr6 polysomy, we performed a hypothesis-generating immunohistochemical analysis and found an association between VEGFA gene copy number amplification or Chr6 polysomy and reduced number of M1 and M2 macrophages, reduced PD-1-expressing lymphocyte infiltration, as well as reduced stromal expression of PD-L1 at the tumor front. The gene discussed is CD274; the disease is neoplasm.