Specimens from 15 patients with soft tissue sarcomas other than ASPS were also processed on ApoStream® at crossover frequency of 85 kHz, infusion rate of 35 μL/min, and effluent rate of 15 μL/min; CTCs were identified using a multiplex phenotyping assay containing a cocktail of antibodies to CTC biomarkers vimentin (VIM), cytokeratin (CK), and β-catenin (β-cat), while excluding CD45+ cells. The gene discussed is VIM; the disease is soft tissue sarcoma.