Apilimod also had no apparent direct effect on the activity of cathepsin B and L (Fig 8), in contrast to EST, a known inhibitor of cathepsin B, H, and L. Several cationic amphiphilic drugs such as U18666A that block EBOV entry and infection [20–22,24,25,68] induce cholesterol accumulation in endolysosomes [20,24]. This evidence concerns the gene CTSB and infection.