The number of glypican-3 positive cells was highest in alcohol-related HCCs (100%), followed by 81% (13/16) in HCV-related HCC, 70% (7/10) in HBV-related HCC and 66% (8/12) in HCC related to NASH (Figure 5, Supplementary Table 1). Here, GPC3 is linked to hepatocellular carcinoma.