To confirm the correlation between PLCE1 and PRKCA in vitro, we used siRNA targeting human PLCE1 in esophageal squamous cell carcinoma cell line EC9706 cells and found that PRKCA protein expression was downregulated once PLCE1 was knocked down (Figure 4C), compared to the scramble control group (negative control, NC), assayed by western blotting. This evidence concerns the gene PRKCA and esophageal squamous cell carcinoma.