PLCE1 and esophageal squamous cell carcinoma: As reported previously and as shown in Figure 5, PLCE1 was slightly expressed in normal esophageal epithelial cells, mainly located at the basal layers and proliferation zone, but the expression was increased in cancer-in-situ, and was highly expressed in ESCC, further, poor-differentiated ESCC showed strongest staining of PLCE1 staining, PRKCA staining was also gradually increased with the progression of ESCC.