We have previously reported that PLCE1 expression levels were increased in limited number of ESCC samples [24], herein, we used an ESCC tissues microarray (TMA) that contained 362 cases of esophageal squamous cell carcinoma with complete clinical information and follow-up results, including some cancer-in-situ and early invasive ESCC, and matched normal mucosa, esophagitis, and conducted immunohistochemical staining with anti-human PLCE1 and PRKCA antibodies, respectively. This evidence concerns the gene PRKCA and esophageal squamous cell carcinoma.