To functionally assess if the co-expanded CD4+CD25hiFoxP3+ T cells were responsible for the low expansion capacity of tumor-specific T cells, we first depleted the CD4+CD25hiFoxP3+ T cells from a T cell infusion product from a non-responder patient with a high CD4+CD25hiFoxP3+ T cell frequency and compared the proliferation of the CD4+CD25hiFoxP3+ T cell-depleted T cell batch to the non-CD4+CD25hiFoxP3+ T cell-depleted T cells during MLTC. Here, CD4 is linked to neoplasm.