LGALS3 and neoplasm: To validate these findings, we used the galectin-3 knock-out cell line to induce tumor-reactive T cells in a MLTC, and this resulted in a marked increase in the expansion of T cells (Fig. 5f), and also a significant increase of IFNγ secretion by tumor-reactive T cells after stimulation with the autologous tumor cells (Fig. 5g) and an increased frequency of tumor-specific CD8+ T cells (Fig. 5h).