To determine which elements downstream of PI3K were required for H. pylori-mediated HIF-1α induction, cells were incubated with the mTOR inhibitor Rapamycin (50 nM) during infection by 4 h or 8 h and mTOR inhibition was assessed by determining the levels of S6 phosphorylation on S235/236 (p-S235/236-S6) due to S6K, a direct target of mTOR (Semenza, 2012). This evidence concerns the gene HIF1A and infection.