Expression levels of Rab25 mRNA were both significantly higher in PCa tissues and LNCaP cells than those in adjacent non-cancerous prostate tissues and PrEC cells (cancer vs. normal: 3.19 ± 1.03 vs. 1.15 ± 0.51, P < 0.001; LNCaP vs. PrEC: 2.90 ± 0.51 vs. 1.09 ± 0.07, P = 0.004; Fig. 1a), which was consistent with the data on Rab25 protein obtained from Western blot analysis (cancer vs. normal: 2.89 ± 0.96 vs. 0.95 ± 0.50, P < 0.001; LNCaP vs. PrEC: 3.08 ± 0.43 vs. 1.09 ± 0.07, P = 0.001, Fig. 1b). Here, RAB25 is linked to cancer.