To examine whether MEG3 affects the expression of DNMT3A by promoting hypophosphorylation of RB at S249/T252, we determined the expression levels of DNMT3A in AML cells after silencing or overexpressing MEG3. The results showed that both mRNA and protein expression levels of DNMT3A negatively correlated with MEG3 abundances (Figures 2m and n; Supplementary Figures 2H and I). This evidence concerns the gene MEG3 and acute myeloid leukemia.