For immunohistochemical analysis, estrogen receptor alpha (ERα, 1:200, anti-estrogen receptor alpha antibody [E115], Abcam, Korea) and progesterone receptor (PR, 1:200, anti-progesterone receptor antibody [PR-AT 4.14], Abcam, Korea) were used in three mammary tumors and testis. This evidence concerns the gene ESR1 and breast cancer.