At 14 dpi, the percentage of apoptotic CD4+ single-positive (SP) T cells were no different between the groups (Fig. 4A), apoptotic CD8+ SP thymocytes increased by 7% in LPi animals due to the interaction of diet plus infection (Fig. 4B) and apoptotic DP cells increased modestly by ~5% in protein restricted animals at 14 dpi (Fig. 4C). This evidence concerns the gene CD8A and infection.