EGFR and triple-negative breast carcinoma: EGFR-activating mutations and oxidative stress morphologically transform MCF-10A cells and enhance their oncogenic properties.37, 41 EGFR overexpression and an EGFR-activating mutation (L858R) have been observed in triple-negative breast cancer (TNBC).42 EGFRwt and the L858R mutant were introduced into MCF-10A cells to study whether EGFRwt or the L858R mutant affect ROS generation in normal breast epithelial cells (Figure 3c).