Furthermore, using live-cell imaging of a Src Fluorescence Resonance Energy Transfer (Src-FRET) biosensor, we show that when co-cultured with endothelial cells, metastatic melanoma cells use cytokines and receptor-ligand interactions to induce activation of Src and VE-cadherin phosphorylation in endothelial cells. Here, CDH5 is linked to metastatic melanoma.