In this study, we demonstrated that S-post significantly increased the expression of p-JAK2 and p-STAT3, which markedly decreased the myocardial infarction area, improved cardiac function indicators and mitochondrial ultrastructure, regulated downstream ROS levels, suppressed myocardial cell apoptosis and the cardioprotective effects of S-post were abolished by the JAK2 selective inhibitor. Here, STAT3 is linked to myocardial infarction.