Included in these isolates was the stx2c-only NSFO157 isolate from a 66-year-old patient and an O111 isolate recovered from an HUS patient with multi-strain infection where one strain was nleH1–2-positive (carrying stx1a and stx2a) and the other strain was negative (stx1a only). This evidence concerns the gene STX2 and hemolytic-uremic syndrome.