All isolates from HUS cases (n = 32) were positive for eae and IpfAO26, and none were positive for saa, eibG, astA, cnf, subA and pic. None of the isolates recovered from cases aged between 20 and 60 years (n = 117), were serotyped as O103:H2 (n = 47), positive for stx2b (n = 32), stx2d (n = 3), stx2g (n = 2), stx1c (n = 23) or stx1d (n = 3), or recovered from cases that developed HUS. This evidence concerns the gene STX2 and hemolytic-uremic syndrome.