To characterize the correlation between methylation of NDRG2 promoter and clinical features of PAs, several clinicopathological characteristics including age, gender, relapse, invasiveness, diagnoses of acromegaly, prolactinoma and Cushing syndrome were compared between methylated and unmethylated NDRG2 promoter groups. This evidence concerns the gene NDRG2 and Cushing syndrome due to macronodular adrenal hyperplasia.