As we know, Acetylcholinesterase was used to decompose acetylcholine, and low Acetylcholinesterase may lead to the accumulation of acetylcholine in the hepatocellular carcinoma, and our current study suggested that high intensity of Acetylcarnitine was associated with poor survival, which indicated that high Acetylcarnitine might promote the release of acetylcholine in ovarian cancer. This evidence concerns the gene ACHE and hepatocellular carcinoma.