Furthermore, the serological cross-reactivity of Zika-positive serum with other flaviviruses, including dengue, Japanese encephalitis, and West Nile viruses, was investigated using DENV Detect IgM capture ELISA, JE Detect IgM capture ELISA, and West Nile Detect IgM capture ELISA (InBios, Seattle, WA, USA), respectively. The gene discussed is CD40LG; the disease is Japanese encephalitis.