Further analysis between the expression of Six1 (cytoplasm and nucleus) and clinical and histopathological data revealed no significant association of those parameters: tumour stage (p = 1.00 and 0.40, respectively), lymph node status (0.29 and 0.48, respectively), tumour grading (0.95 and 0.19, respectively) (Table 1 and Additional file 1: Table S1). Here, SIX1 is linked to neoplasm.