MYL9 expression levels in the nine ESCC cell lines (KYSE30, KYSE180, KYSE140, KYSE410, KYSE510, KYSE520, HKESC1, EC18, EC109) were assessed and compared with NE1 cells by western blotting and qPCR. The gene discussed is MYL9; the disease is esophageal squamous cell carcinoma.