Similarly, the frequency of carriers of at least one S -allele was higher in CD patients with isolated colonic disease (17.8%) and in CD patients needing immunosuppressive therapy (16.7%), especially anti-TNF-alpha agents (18.8%), than in CD patients with no need for immunosuppressive therapy, but this differences were also not significant after correction for multiple testing. The gene discussed is TNF; the disease is colonic disorder.