Given the importance of multifunctional effector CD8+ T cell immunity in anti-tumor immunity [30–32], we measured the Ag-specific CD8+ T cell population and its expression of IFNγ and TNFα, in response to ex vivo OVA257-264 SIINFEKL peptide stimulation, 15 days after tumor implantation (Figure 3A). This evidence concerns the gene TNF and neoplasm.