The majority of CD4+ T cells in HD-PBMC, PBC-PBMC and NILs did not express either FoxP3 or Helios, and had very few FoxP3/Helios co-expressing cells, whereas the relative proportion of FoxP3+Helios+ Tregs was significantly higher in TILs (Figure 6G). This evidence concerns the gene IKZF2 and Huntington disease.