First, exploiting the observation that EMT increases the proportion of CSC-like cells ( ̃100-fold) within breast cancer cell populations, we employed V12H-RAS-transformed derivatives of immortalized mammary epithelial cells driven to undergo EMT by E-cadherin knockdown to assay the ability of denosumab to selectively reduce the EMT-driven enrichment of CSC-like cells [22–26]. Here, CDH1 is linked to breast carcinoma.