Bicyclol treatment exhibited significant protection against anti-TB drug induced liver damage by attenuating the elevated ALT, AST, AKP, and TBIL levels in a dose-dependent manner, while histopathological changes including nuclear pleomorphism, increased cellular size, degeneration in hepatocytes and hepatic cords, cytoplasm dissolution, and inflammatory cell infiltration were also remarkably improved by bicyclol treatment. This evidence concerns the gene GPT and tuberculosis.